Identification using phage display of peptides promoting targeting and internalization into HPV‐transformed cell lines
Identifieur interne : 001E69 ( Main/Exploration ); précédent : 001E68; suivant : 001E70Identification using phage display of peptides promoting targeting and internalization into HPV‐transformed cell lines
Auteurs : Philip Robinson [France] ; Denise Stuber [France] ; François Deryckère [France] ; Philip Tedbury [France] ; Magali Lagrange [France] ; Georges Orfanoudakis [France]Source :
- Journal of Molecular Recognition [ 0952-3499 ] ; 2005-03.
English descriptors
- Teeft :
- Alexa fluor1, Amersham biosciences, Amino, Amino acid sequence, Biopanning, Bioscience, Carcinoma, Caski, Caski cells, Cell line, Cell lines, Cell surface, Cervical, Cervical cancer, Chloroquine, Clone, Copyright, Disulphide bond, Egfp, Focal plane medial, Fusion products, Hela, Hela cells, Human papillomavirus, Human transferrin, Individual clones, Internalization, Internalizing, Internalizing peptides, Invitrogen, Ivanenkov, John wiley sons, Keratinocytes, Mammalian cells, Other cell lines, Papillomavirus, Peptide, Phage, Phage display, Phage display technology, Phage particles, Phagemid, Phagemid library, Pviii, Receptor, Recognit, Room temperature, Sequence coding, Siha, Siha cells, Target cells, Transferrin, Tumour.
Abstract
‘High‐risk’ human papilloma viruses (HPVs) cause cervical tumours. In order to treat these tumours therapeutic approaches must be developed that efficiently target the tumour cells. Using phage display, we selected tumour‐targeting peptides from a library of constrained nonamer peptides presented multivalently on pVIII of M13. Three different consensus peptide sequences were isolated by biopanning on HPV16‐transformed SiHa cells. The corresponding phage‐peptides targeted and were internalized in HPV16 transformed SiHa and CaSki cells as well as in HPV18‐transformed HeLa cells, but failed to bind a panel of normal or transformed cell lines. Two of the three selected peptides targeted cells only when presented on phage particles in a constrained conformation. However, all three peptides retained their targeting capacity when presented on the reporter protein enhanced green fluorescent protein (EGFP) in a monovalent form. These peptides may be useful for the design of drug or gene delivery vectors for the treatment of cervical cancer. Copyright © 2004 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jmr.723
Affiliations:
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In order to treat these tumours therapeutic approaches must be developed that efficiently target the tumour cells. Using phage display, we selected tumour‐targeting peptides from a library of constrained nonamer peptides presented multivalently on pVIII of M13. Three different consensus peptide sequences were isolated by biopanning on HPV16‐transformed SiHa cells. The corresponding phage‐peptides targeted and were internalized in HPV16 transformed SiHa and CaSki cells as well as in HPV18‐transformed HeLa cells, but failed to bind a panel of normal or transformed cell lines. Two of the three selected peptides targeted cells only when presented on phage particles in a constrained conformation. However, all three peptides retained their targeting capacity when presented on the reporter protein enhanced green fluorescent protein (EGFP) in a monovalent form. These peptides may be useful for the design of drug or gene delivery vectors for the treatment of cervical cancer. Copyright © 2004 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><orgName><li>Université Strasbourg 1</li></orgName></list><tree><country name="France"><region name="Grand Est"><name sortKey="Robinson, Philip" sort="Robinson, Philip" uniqKey="Robinson P" first="Philip" last="Robinson">Philip Robinson</name></region><name sortKey="Deryckere, Francois" sort="Deryckere, Francois" uniqKey="Deryckere F" first="François" last="Deryckère">François Deryckère</name><name sortKey="Lagrange, Magali" sort="Lagrange, Magali" uniqKey="Lagrange M" first="Magali" last="Lagrange">Magali Lagrange</name><name sortKey="Orfanoudakis, Georges" sort="Orfanoudakis, Georges" uniqKey="Orfanoudakis G" first="Georges" last="Orfanoudakis">Georges Orfanoudakis</name><name sortKey="Orfanoudakis, Georges" sort="Orfanoudakis, Georges" uniqKey="Orfanoudakis G" first="Georges" last="Orfanoudakis">Georges Orfanoudakis</name><name sortKey="Orfanoudakis, Georges" sort="Orfanoudakis, Georges" uniqKey="Orfanoudakis G" first="Georges" last="Orfanoudakis">Georges Orfanoudakis</name><name sortKey="Stuber, Denise" sort="Stuber, Denise" uniqKey="Stuber D" first="Denise" last="Stuber">Denise Stuber</name><name sortKey="Tedbury, Philip" sort="Tedbury, Philip" uniqKey="Tedbury P" first="Philip" last="Tedbury">Philip Tedbury</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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